Identification of a New Acanthamoeba 18S rRNA Gene Sequence Type, Corresponding to the Species Acanthamoeba jacobsi Sawyer, Nerad and Visvesvara, 1992 (Lobosea: Acanthamoebidae)
نویسندگان
چکیده
Since 1996, a new classification system has been used to assign members of the genus Acanthamoeba to different sequence types. This study aimed to place the thermophilic species Acanthamoeba jacobsi into the sequence type classification by sequencing part of the 18S rRNA gene for six isolates of this species. Phylogenetic analysis of the sequences showed the six strains to be closely related and confirmed their identification as A. jacobsi. This species is well separated from all previously typed Acanthamoeba strains, and is allocated to a new sequence type, T15.
منابع مشابه
The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types.
The 18S rRNA gene (Rns) phylogeny of Acanthamoeba is being investigated as a basis for improvements in the nomenclature and taxonomy of the genus. We previously analyzed Rns sequences from 18 isolates from morphological groups 2 and 3 and found that they fell into four distinct evolutionary lineages we called sequence types T1-T4. Here, we analyzed sequences from 53 isolates representing 16 spe...
متن کاملFluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type.
The first genus- and subgenus-specific fluorescent oligonucleotide probes for in situ staining of Acanthamoeba are described. Sequences of these phylogeny-based probes complement the 18S rRNA and the gene encoding it (18S rDNA). The genus-specific probe (GSP) is a fluorescein-labeled 22-mer specific for Acanthamoeba as shown here by its hybridization to growing trophozoites of all 12 known Acan...
متن کاملشیوع آکانتامبا در آبهای سطحی شهر تهران
Abstract: Background: Acanthamoeba is a genus of amoeba, one of the most common protozoa found worldwide in soil, and also frequently found in fresh water. In healthy individuals, Acanthamoeba spp. can cause ulcerating keratitis which is often associated with the use of improperly sterilized contact lenses. The aim of this study was to detect Acanthamoeba from fresh water collected from some t...
متن کاملSpecies Identification of Acanthamoeba Strains Isolated from Patients Referring to Farabi Eye Reference Center Using PCR-RFLP Method
Introduction: Acanthamoeba is an opportunistic protist, which is ubiquitously distributed in the environment. Infection with Acanthamoeba spp. poses threat to human health, such as, Acanthamoeba keratitis (AK) that is a vision-threatening infection of the cornea. This study aimed to identify the species of Acanthamoeba strains isolated from cornea of keratitis patients by polymerase chain react...
متن کاملAcanthamoeba Encephalitis in Patient with Systemic Lupus, India
We report a fatal case of encephalitis caused by Acanthamoeba in a 24-year-old woman from India with systemic lupus erythematosus. Diagnosis was made by identification of amebas in brain sections by immunofluorescence analysis and confirmed by demonstrating Acanthamoeba mitochondrial 16S rRNA gene DNA in brain tissue sections.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2003